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rabbit antibody against p35 p25  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit antibody against p35 p25
Fig. 3. Aβ25-35 induces <t>p25</t> production from cleavage of <t>p35</t> without affecting CDK5 expression in cultured cortical neurons. Primary cortical cultures were treated with 10 μM Aβ25-35 for indicated times before detection of p35/p25 (A) or CDK5 (E) by Western blotting. The β-actin in (A) and α-tubulin in (E) served as the respective internal control for equal loading of proteins in each lane. Quantitative analyses of the signal intensities on the blots for p25, p35, p25/p35 ratio, and CDK5 are shown respectively in (B), (C), (D), and (F). Mean ± S.E.M. from N = 3 in all the statistical analyses. * denotes P < 0.05 compared with the corresponding control cultures without Aβ25-35 treatment in (B)–(D). The “ns” in (F) denotes “not significant”.
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Fig. 3. Aβ25-35 induces p25 production from cleavage of p35 without affecting CDK5 expression in cultured cortical neurons. Primary cortical cultures were treated with 10 μM Aβ25-35 for indicated times before detection of p35/p25 (A) or CDK5 (E) by Western blotting. The β-actin in (A) and α-tubulin in (E) served as the respective internal control for equal loading of proteins in each lane. Quantitative analyses of the signal intensities on the blots for p25, p35, p25/p35 ratio, and CDK5 are shown respectively in (B), (C), (D), and (F). Mean ± S.E.M. from N = 3 in all the statistical analyses. * denotes P < 0.05 compared with the corresponding control cultures without Aβ25-35 treatment in (B)–(D). The “ns” in (F) denotes “not significant”.

Journal: Biochimica et biophysica acta. Molecular cell research

Article Title: Roles of Id1/HIF-1 and CDK5/HIF-1 in cell cycle reentry induced by amyloid-beta peptide in post-mitotic cortical neuron.

doi: 10.1016/j.bbamcr.2019.118628

Figure Lengend Snippet: Fig. 3. Aβ25-35 induces p25 production from cleavage of p35 without affecting CDK5 expression in cultured cortical neurons. Primary cortical cultures were treated with 10 μM Aβ25-35 for indicated times before detection of p35/p25 (A) or CDK5 (E) by Western blotting. The β-actin in (A) and α-tubulin in (E) served as the respective internal control for equal loading of proteins in each lane. Quantitative analyses of the signal intensities on the blots for p25, p35, p25/p35 ratio, and CDK5 are shown respectively in (B), (C), (D), and (F). Mean ± S.E.M. from N = 3 in all the statistical analyses. * denotes P < 0.05 compared with the corresponding control cultures without Aβ25-35 treatment in (B)–(D). The “ns” in (F) denotes “not significant”.

Article Snippet: The mouse antibody against β-actin (1:4000; Cat. No. MAB1501; Merck Millipore), mouse antibody against α-tubulin (1:10000; Cat. No. T9026; Sigma-Aldrich), rabbit antibody against cyclin D1 (1:1000; Cat. No. ab134175; Abcam), rabbit antibody against PCNA (1:500; Cat. No. ab92552; Abcam), rabbit antibody against HIF-1α (1:500; Cat. No. NB100-134; Novus Biologicals, Littleton, CO, USA), rabbit antibody against CDK5 (1:500; Cat. No. ab40773; Abcam), and rabbit antibody against p35/p25 (1:500; Cat. No. 2680; Cell Signaling Technology, Beverly, MA, USA) were all diluted in blocking buffer (5% nonfat dry milk in TBST buffer containing 0.05% Tween 20, 137 mM NaCl, and 20 mM Tris–HCl, pH 7.5).

Techniques: Expressing, Cell Culture, Western Blot, Control

Fig. 5. Suppression of Id1 enhances p25 production, whereas exogenous Id1-Tag suppresses p25 production in rat cortical cultures. (A–F) Primary cortical cultures were transfected with sc siRNA or Id1 siRNA for 72 h followed by exposure to 10 μM Aβ25-35 for additional 4 h before detection of Id1 (A) or p35/p25 (C) by Western blotting. Quantitative analyses of the signal intensities on the blots for Id1, p25, p25/p35 ratio, and p35 are respectively shown in (B), (D), (E), and (F). Mean ± S.E.M. from N = 3 in all the statistical analyses. * and # denote P < 0.05. The “ns” denotes “not significant”. (G–L) Primary cortical cultures were treated with or without 4 μg/ml Id1-Tag for 2 h or 4 h before detection of p35/p25 (G) or CDK5 (K). Quantitative analyses of the signal intensities on the blots for p25, p25/ p35 ratio, p35, and CDK5 are shown respectively in (H), (I), (J), and (L). Mean ± S.E.M. from N = 4 in 2 h and N = 5 in 4 h. * denotes P < 0.05 as compared to the corresponding control cultures. The “ns” denotes “not significant”.

Journal: Biochimica et biophysica acta. Molecular cell research

Article Title: Roles of Id1/HIF-1 and CDK5/HIF-1 in cell cycle reentry induced by amyloid-beta peptide in post-mitotic cortical neuron.

doi: 10.1016/j.bbamcr.2019.118628

Figure Lengend Snippet: Fig. 5. Suppression of Id1 enhances p25 production, whereas exogenous Id1-Tag suppresses p25 production in rat cortical cultures. (A–F) Primary cortical cultures were transfected with sc siRNA or Id1 siRNA for 72 h followed by exposure to 10 μM Aβ25-35 for additional 4 h before detection of Id1 (A) or p35/p25 (C) by Western blotting. Quantitative analyses of the signal intensities on the blots for Id1, p25, p25/p35 ratio, and p35 are respectively shown in (B), (D), (E), and (F). Mean ± S.E.M. from N = 3 in all the statistical analyses. * and # denote P < 0.05. The “ns” denotes “not significant”. (G–L) Primary cortical cultures were treated with or without 4 μg/ml Id1-Tag for 2 h or 4 h before detection of p35/p25 (G) or CDK5 (K). Quantitative analyses of the signal intensities on the blots for p25, p25/ p35 ratio, p35, and CDK5 are shown respectively in (H), (I), (J), and (L). Mean ± S.E.M. from N = 4 in 2 h and N = 5 in 4 h. * denotes P < 0.05 as compared to the corresponding control cultures. The “ns” denotes “not significant”.

Article Snippet: The mouse antibody against β-actin (1:4000; Cat. No. MAB1501; Merck Millipore), mouse antibody against α-tubulin (1:10000; Cat. No. T9026; Sigma-Aldrich), rabbit antibody against cyclin D1 (1:1000; Cat. No. ab134175; Abcam), rabbit antibody against PCNA (1:500; Cat. No. ab92552; Abcam), rabbit antibody against HIF-1α (1:500; Cat. No. NB100-134; Novus Biologicals, Littleton, CO, USA), rabbit antibody against CDK5 (1:500; Cat. No. ab40773; Abcam), and rabbit antibody against p35/p25 (1:500; Cat. No. 2680; Cell Signaling Technology, Beverly, MA, USA) were all diluted in blocking buffer (5% nonfat dry milk in TBST buffer containing 0.05% Tween 20, 137 mM NaCl, and 20 mM Tris–HCl, pH 7.5).

Techniques: Transfection, Western Blot, Control

Fig. 7. A diagram showing the proposed signal transduction pathway. We propose possible molecular mechanisms un- derlying Aβ25-35-dependent neuronal cell cycle reentry through Id1- and CDK5/p25-dependent HIF-1α induction in the fully differentiated post-mitotic cortical neurons. This mechanism is further complicated by the intriguing antag- onism between Id1 and p25 because they reciprocally sup- press expression of each other. Possible involvements of unknown mediators, as indicated by a red question mark, linking Aβ and HIF-1 is also shown. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Biochimica et biophysica acta. Molecular cell research

Article Title: Roles of Id1/HIF-1 and CDK5/HIF-1 in cell cycle reentry induced by amyloid-beta peptide in post-mitotic cortical neuron.

doi: 10.1016/j.bbamcr.2019.118628

Figure Lengend Snippet: Fig. 7. A diagram showing the proposed signal transduction pathway. We propose possible molecular mechanisms un- derlying Aβ25-35-dependent neuronal cell cycle reentry through Id1- and CDK5/p25-dependent HIF-1α induction in the fully differentiated post-mitotic cortical neurons. This mechanism is further complicated by the intriguing antag- onism between Id1 and p25 because they reciprocally sup- press expression of each other. Possible involvements of unknown mediators, as indicated by a red question mark, linking Aβ and HIF-1 is also shown. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The mouse antibody against β-actin (1:4000; Cat. No. MAB1501; Merck Millipore), mouse antibody against α-tubulin (1:10000; Cat. No. T9026; Sigma-Aldrich), rabbit antibody against cyclin D1 (1:1000; Cat. No. ab134175; Abcam), rabbit antibody against PCNA (1:500; Cat. No. ab92552; Abcam), rabbit antibody against HIF-1α (1:500; Cat. No. NB100-134; Novus Biologicals, Littleton, CO, USA), rabbit antibody against CDK5 (1:500; Cat. No. ab40773; Abcam), and rabbit antibody against p35/p25 (1:500; Cat. No. 2680; Cell Signaling Technology, Beverly, MA, USA) were all diluted in blocking buffer (5% nonfat dry milk in TBST buffer containing 0.05% Tween 20, 137 mM NaCl, and 20 mM Tris–HCl, pH 7.5).

Techniques: Transduction, Expressing